Journal: Parasite

Article Title: First genotyping of Giardia duodenalis and prevalence of enteroparasites in children from Tetouan (Morocco)

doi: 10.1051/parasite/2014049

Figure Lengend Snippet: Multiple infections by enteroparasites in children of Tetouan (Morocco).

Article Snippet: Lane 1, molecular-weight marker (1.000 bp); lane 2, positive control of Giardia duodenalis (ATCC 30888); lanes 3–13, polymerase chain reaction products from examined samples.

Techniques:

Journal: Parasite

Article Title: First genotyping of Giardia duodenalis and prevalence of enteroparasites in children from Tetouan (Morocco)

doi: 10.1051/parasite/2014049

Figure Lengend Snippet: PCR amplification of Giardia duodenalis gdh on 2% agarose gel stained with Ethidium Bromide. Lane 1, molecular-weight marker (1.000 bp); lane 2, positive control of Giardia duodenalis (ATCC 30888); lanes 3–13, polymerase chain reaction products from examined samples.

Article Snippet: Lane 1, molecular-weight marker (1.000 bp); lane 2, positive control of Giardia duodenalis (ATCC 30888); lanes 3–13, polymerase chain reaction products from examined samples.

Techniques: Amplification, Agarose Gel Electrophoresis, Staining, Molecular Weight, Marker, Positive Control, Polymerase Chain Reaction

Journal: Parasite

Article Title: First genotyping of Giardia duodenalis and prevalence of enteroparasites in children from Tetouan (Morocco)

doi: 10.1051/parasite/2014049

Figure Lengend Snippet: PCR amplification of Giardia duodenalis SSUrRNA on 2% agarose gel stained with Ethidium Bromide. Lane 1, molecular-weight marker (1.000 bp); lane 2–12 polymerase chain reaction products from examined samples; lane 13, positive control of Giardia duodenalis (ATCC 30888).

Article Snippet: Lane 1, molecular-weight marker (1.000 bp); lane 2, positive control of Giardia duodenalis (ATCC 30888); lanes 3–13, polymerase chain reaction products from examined samples.

Techniques: Amplification, Agarose Gel Electrophoresis, Staining, Molecular Weight, Marker, Polymerase Chain Reaction, Positive Control

Journal: ACS Infectious Diseases

Article Title: Host–Pathogen Cellular Communication: The Role of Dynamin, Clathrin, and Macropinocytosis in the Uptake of Giardia-Derived Extracellular Vesicles

doi: 10.1021/acsinfecdis.4c00996

Figure Lengend Snippet: Characterization of extracellular vesicles isolated during host–parasite interaction. (A) Schematic representation of the differential centrifugation process used to isolate different types of EVs: G. intestinalis -derived EVs (gEVs), host-derived EVs (hEVs), and EVs resulting from host–parasite interaction (intEVs). (B) Particle size distribution analyzed by nanoparticle tracking analysis (NTA). (C) Protein concentration of gEVs, hEVs, and intEVs measured using a protein-based assay.

Article Snippet: The G. intestinalis WB isolate (ATCC 50803) was cultured in a TYI-S-33 medium, supplemented with 10% heat-inactivated adult bovine serum (ABS) and 1% penicillin/streptomycin (1000 U/mL, Gibco), at 37 °C under microaerophilic conditions.

Techniques: Isolation, Centrifugation, Derivative Assay, Protein Concentration

Journal: ACS Infectious Diseases

Article Title: Host–Pathogen Cellular Communication: The Role of Dynamin, Clathrin, and Macropinocytosis in the Uptake of Giardia-Derived Extracellular Vesicles

doi: 10.1021/acsinfecdis.4c00996

Figure Lengend Snippet: EVs from the parasite–host cell interaction (intEV) are rapidly acquired by intestinal cells. (A) Confocal microscopy images comparing the internalization of G. intestinalis -derived EVs (gEVs), host–derived EVs (hEVs), and EVs resulting from host–parasite interaction (intEVs) in Caco-2 cells after 1 and 3 h of incubation. The control shows no EV signal. Internalized EVs are highlighted in green (PKH67), while cell nuclei are shown in blue (DAPI), and the cytoskeleton in red (phalloidin). (B) Quantification of EV uptake based on fluorescence intensity after 1 and 3 h. Scale bar = 10 μm.

Article Snippet: The G. intestinalis WB isolate (ATCC 50803) was cultured in a TYI-S-33 medium, supplemented with 10% heat-inactivated adult bovine serum (ABS) and 1% penicillin/streptomycin (1000 U/mL, Gibco), at 37 °C under microaerophilic conditions.

Techniques: Confocal Microscopy, Derivative Assay, Incubation, Control, Fluorescence

Journal: ACS Infectious Diseases

Article Title: Host–Pathogen Cellular Communication: The Role of Dynamin, Clathrin, and Macropinocytosis in the Uptake of Giardia-Derived Extracellular Vesicles

doi: 10.1021/acsinfecdis.4c00996

Figure Lengend Snippet: Uptake of EVs is dynamin and clathrin-dependent on Caco-2 cells. (A) Flow cytometry analysis showing inhibition of G. intestinalis -derived EV (gEV) uptake in the presence of different pathway inhibitors, including methyl-β-cyclodextrin (MβCD), wortmannin, cytochalasin D, dynasore, and control (Ctrl, without an inhibitor). (B) Dose-dependent inhibition of gEV uptake by dynasore at concentrations of 50, 100, and 200 μM. (C, D) Inhibition of host-derived EV (hEV) and interaction-derived EV (intEV) uptake by dynasore at 100 μM. (E) Percentage inhibition of gEV, hEV, and intEV uptake by various endocytosis inhibitors, including bafilomycin A1, EIPA, genistein, MDC, MβCD, and dynasore. (F) Cell viability assay with endocytosis inhibitors, bafilomycin A1, EIPA, genistein, MDC dynasore, and positive control (DMSO 15%).

Article Snippet: The G. intestinalis WB isolate (ATCC 50803) was cultured in a TYI-S-33 medium, supplemented with 10% heat-inactivated adult bovine serum (ABS) and 1% penicillin/streptomycin (1000 U/mL, Gibco), at 37 °C under microaerophilic conditions.

Techniques: Flow Cytometry, Inhibition, Derivative Assay, Control, Viability Assay, Positive Control

Journal: Nanomaterials

Article Title: Impact of Aging on the Ovarian Extracellular Matrix and Derived 3D Scaffolds

doi: 10.3390/nano12030345

Figure Lengend Snippet: ELISA kits used to quantify the different ECM components.

Article Snippet: LAMB1 , Porcine Laminin subunit beta-1 (LAMB1) ELISA Kit (MyBioSource, San Diego, CA, USA) , MBS2613645.

Techniques: Enzyme-linked Immunosorbent Assay, Collagen Assay

Journal: Nanomaterials

Article Title: Impact of Aging on the Ovarian Extracellular Matrix and Derived 3D Scaffolds

doi: 10.3390/nano12030345

Figure Lengend Snippet: List of primers used for quantitative PCR analysis.

Article Snippet: LAMB1 , Porcine Laminin subunit beta-1 (LAMB1) ELISA Kit (MyBioSource, San Diego, CA, USA) , MBS2613645.

Techniques: Real-time Polymerase Chain Reaction

Journal: Nanomaterials

Article Title: Impact of Aging on the Ovarian Extracellular Matrix and Derived 3D Scaffolds

doi: 10.3390/nano12030345

Figure Lengend Snippet: Histochemical and immunohistochemical analysis, stereological quantification, and ELISA tests in young and aged ovaries. ( a ) Masson’s trichrome staining showed the presence of collagen (blue) and elastic (magenta) fibers in both young and aged ovaries. Scale bars = 100 μm; ( b ) Stereological analysis demonstrated a significant increase of collagen fibers in aged ovaries compared to the young ones. Data are expressed as the mean. Error bars represent the standard error of the mean (SEM), ** p < 0.01; ( c ) ELISA quantifications indicated a significant increase of the collagen content in aged ovaries compared to the young ones. Data are expressed as the mean. Error bars represent the standard error of the mean (SEM), ** p < 0.01; ( d ) Gomori’s Aldehyde Fuchsin staining revealed the presence of elastic fibers in both young and aged ovaries. Scale bars = 100 μm; ( e ) Stereological analysis displayed a significant decrease of elastic fibers in aged ovaries compared to the young ones. Data are expressed as the mean. Error bars represent the standard error of the mean (SEM), ** p < 0.01; ( f ) ELISA quantifications confirmed a significant decrease of the elastin content in aged ovaries compared to the young ones. Data are expressed as the mean. Error bars represent the standard error of the mean (SEM), ** p < 0.01; ( g ) Alcian blue staining showed the presence of GAGs in both young and aged ovaries. Scale bars = 100 μm; ( h ) Stereological analysis indicated a significant increase of GAGs in aged ovaries compared to the young ones. Data are expressed as the mean. Error bars represent the standard error of the mean (SEM), * p < 0.05; ( i ) ELISA quantifications confirmed a significant increase of the GAG content in aged ovaries compared to the young ones. Data are expressed as the mean. Error bars represent the standard error of the mean (SEM), * p < 0.05; ( j ) Immunohistochemical staining of young and aged ovaries for LAMA3. Scale bars = 100 μm; ( k ) Stereological analysis demonstrated a significant increase of LAMA3 in aged ovaries compared to the young ones. Data are expressed as the mean. Error bars represent the standard error of the mean (SEM), ** p < 0.01; ( l ) ELISA quantifications showed a significant increase of the LAMA3 content in aged ovaries compared to the young ones. Data are expressed as the mean. Error bars represent the standard error of the mean (SEM), ** p < 0.01; ( m ) Immunohistochemical staining of young and aged ovaries for LAMB1. Scale bars = 100 μm; ( n ) Stereological analysis revealed a significant increase of LAMB1 in aged ovaries compared to the young ones. Data are expressed as the mean. Error bars represent the standard error of the mean (SEM), ** p < 0.01; ( o ) ELISA quantifications displayed a significant increase of the LAMB1 content in aged ovaries compared to the young ones. Data are expressed as the mean. Error bars represent the standard error of the mean (SEM), ** p < 0.01; ( p ) Immunohistochemical staining of young and aged ovaries for FN1. Scale bars = 100 μm; ( q ) Stereological analysis indicated a significant decrease in FN1 content in aged ovaries compared to the young ones. Data are expressed as the mean. Error bars represent the standard error of the mean (SEM), ** p < 0.01; ( r ) ELISA quantifications showed a significant increase of the LAMB1 content in aged ovaries compared to the young ones. Data are expressed as the mean. Error bars represent the standard error of the mean (SEM), ** p < 0.01.

Article Snippet: LAMB1 , Porcine Laminin subunit beta-1 (LAMB1) ELISA Kit (MyBioSource, San Diego, CA, USA) , MBS2613645.

Techniques: Immunohistochemical staining, Enzyme-linked Immunosorbent Assay, Staining

Journal: Nanomaterials

Article Title: Impact of Aging on the Ovarian Extracellular Matrix and Derived 3D Scaffolds

doi: 10.3390/nano12030345

Figure Lengend Snippet: Gene expression changes of extracellular matrix-related genes in young (white bars) and aged ovaries (black bars). Expression levels of collagens (COL1A1, COL3A1, COL4A2), elastin (ELN), fibronectin (FN1), glycoprotein (EMILIN1), laminins (LAMA3, LAMB1), proteoglycans (VCAN, HSPG2, CSPG4), and proteases (ELANE, MMP1, MMP2, MMP3, MMP9, MMP12, and MMP14). Data are expressed as the mean. Error bars represent the standard error of the mean (SEM), * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: LAMB1 , Porcine Laminin subunit beta-1 (LAMB1) ELISA Kit (MyBioSource, San Diego, CA, USA) , MBS2613645.

Techniques: Gene Expression, Expressing

Journal: Nanomaterials

Article Title: Impact of Aging on the Ovarian Extracellular Matrix and Derived 3D Scaffolds

doi: 10.3390/nano12030345

Figure Lengend Snippet: Immunohistochemical characterizations of young and aged ECM-based scaffolds. ( a ) LAMA3 immunostaining displayed the persistence of the fibers after decellularization in both young and aged tissues; Stereological analysis ( b ) and ELISA quantifications (c) demonstrated no significant differences between native (Native) and decellularized (Scaffold) ovaries in LAMA3 content. Data are expressed as the mean. Error bars represent the standard error of the mean (SEM), ** p < 0.01; ( d ) LAMB1 immunostaining showed the maintenance of the fibers at the end of the process in both young and aged organs; Stereological analysis ( e ) and ELISA tests ( f ) indicated no significant differences between native (Native) and decellularized tissues (Scaffold) in LAMB1 content. Data are expressed as the mean. Error bars represent the standard error of the mean (SEM), ** p < 0.01; ( g ) FN1 immunostaining revealed fiber retainment in both young and aged decellularized tissues; Stereological ( h ) and ELISA analysis ( i ) showed no significant differences between native (Native) and decellularized samples (Scaffold) in FN1 content. Data are expressed as the mean. Error bars represent the standard error of the mean (SEM), ** p < 0.01.

Article Snippet: LAMB1 , Porcine Laminin subunit beta-1 (LAMB1) ELISA Kit (MyBioSource, San Diego, CA, USA) , MBS2613645.

Techniques: Immunohistochemical staining, Immunostaining, Enzyme-linked Immunosorbent Assay

Journal: OncoTargets and therapy

Article Title: Upregulated LAMB3 increases proliferation and metastasis in thyroid cancer

doi: 10.2147/OTT.S149613

Figure Lengend Snippet: LAMB3 relative expression was significantly upregulated in thyroid cancer samples compared with normal thyroid tissues in validated cohort and TCGA cohort. Notes: ( A ) LAMB3 expression was upregulated by 19 pairs of matched thyroid cancer and normal tissues by RNA-seq (*** P <0.01). ( B ) LAMB3 relative expression was validated by 59 pairs of matched thyroid cancer and normal tissues by TGCA database (*** P <0.01). ( C ) LAMB3 expression was examined in 56 paired PTC samples with adjacent normal tissues and 33 benign thyroid nodules with adjacent normal tissues by RT-qPCR (*** P <0.01). ( D ) LAMB3 expression level for differentiating normal tissue from PTC in our validated cohort (59 pairs of PTC tissues with adjacent normal tissues); AUC: 97.3%, sensitivity: 94.6%, and specificity: 98.2% in the validated cohort. ( E ) LAMB3 expression level for differentiating normal tissue from PTC in TCGA cohort (507 PTC tissues and 59 normal tissues), AUC: 90.1%, sensitivity: 83.1%, and specificity: 91.5% in TCGA cohort. Abbreviations: AUC, area under the curve; ns, no sense; PTC, papillary thyroid cancer; RNA-seq, RNA sequencing; RT-qPCR, real-time quantitative polymerase chain reaction; TPM, transcripts per million; TCGA, The Cancer Genome Atlas.

Article Snippet: Small interfering RNA (siRNA) for LAMB3 and nontargeting siRNA (negative control [NC] siRNA) were purchased from GenePharma (Shanghai, People’s Republic of China) for siRNA-mediated gene knockdown.

Techniques: Expressing, RNA Sequencing, Quantitative RT-PCR, Real-time Polymerase Chain Reaction

Journal: OncoTargets and therapy

Article Title: Upregulated LAMB3 increases proliferation and metastasis in thyroid cancer

doi: 10.2147/OTT.S149613

Figure Lengend Snippet: The relationship between LAMB3 expression and clinicopathologic features in TCGA cohort

Article Snippet: Small interfering RNA (siRNA) for LAMB3 and nontargeting siRNA (negative control [NC] siRNA) were purchased from GenePharma (Shanghai, People’s Republic of China) for siRNA-mediated gene knockdown.

Techniques: Expressing

Journal: OncoTargets and therapy

Article Title: Upregulated LAMB3 increases proliferation and metastasis in thyroid cancer

doi: 10.2147/OTT.S149613

Figure Lengend Snippet: The relationship between LAMB3 expression and clinicopathologic features in the validated cohort

Article Snippet: Small interfering RNA (siRNA) for LAMB3 and nontargeting siRNA (negative control [NC] siRNA) were purchased from GenePharma (Shanghai, People’s Republic of China) for siRNA-mediated gene knockdown.

Techniques: Expressing

Journal: OncoTargets and therapy

Article Title: Upregulated LAMB3 increases proliferation and metastasis in thyroid cancer

doi: 10.2147/OTT.S149613

Figure Lengend Snippet: Univariate logistic regression analysis for the lymph node metastatic risk

Article Snippet: Small interfering RNA (siRNA) for LAMB3 and nontargeting siRNA (negative control [NC] siRNA) were purchased from GenePharma (Shanghai, People’s Republic of China) for siRNA-mediated gene knockdown.

Techniques: Expressing

Journal: OncoTargets and therapy

Article Title: Upregulated LAMB3 increases proliferation and metastasis in thyroid cancer

doi: 10.2147/OTT.S149613

Figure Lengend Snippet: Multivariate logistic regression analysis for the lymph node metastatic risk

Article Snippet: Small interfering RNA (siRNA) for LAMB3 and nontargeting siRNA (negative control [NC] siRNA) were purchased from GenePharma (Shanghai, People’s Republic of China) for siRNA-mediated gene knockdown.

Techniques: Expressing

Journal: OncoTargets and therapy

Article Title: Upregulated LAMB3 increases proliferation and metastasis in thyroid cancer

doi: 10.2147/OTT.S149613

Figure Lengend Snippet: Knockdown of LAMB3 gene inhibited proliferation and colony formation in PTC cell lines. Notes: ( A ) LAMB3 relative expression was detected in TPC1, BCPAP, and KTC-1 cell lines transfected with siRNA by RT-qPCR (*** P <0.01). ( B ) Cell proliferation assay. TPC1, BCPAP, and KTC-1 cells transfected with siRNA or NC cells were cultured in 96-well plates for the successive time (1–5 days), and cell proliferation was measured using CCK-8. Cell proliferation was significantly suppressed in TPC1, BCPAP, and KTC-1 cells transfected with siRNA compared with NC. ( C ) Colony formation assay. TPC1, BCPAP, and KTC-1 cells transfected with siRNA and NC cells were plated in six-well plates at the proper density. After 8–14 days of incubation, cells were fixed with 4% paraformaldehyde and stained with 0.01% crystal violet. ( D ) Relative quantification of the colony number. The columns represent the mean colony number from at least three independent experiments, and the little vertical bars on the top of the columns represent SD. ** P <0.05, *** P <0.01 in comparison with NC using Student’s t -test. Abbreviations: CCK-8, Cell Counting Kit-8; NC, negative control; PTC, papillary thyroid cancer; RT-qPCR, real-time quantitative polymerase chain reaction; siRNA, small interfering RNA.

Article Snippet: Small interfering RNA (siRNA) for LAMB3 and nontargeting siRNA (negative control [NC] siRNA) were purchased from GenePharma (Shanghai, People’s Republic of China) for siRNA-mediated gene knockdown.

Techniques: Knockdown, Expressing, Transfection, Quantitative RT-PCR, Proliferation Assay, Cell Culture, CCK-8 Assay, Colony Assay, Incubation, Staining, Quantitative Proteomics, Comparison, Cell Counting, Negative Control, Real-time Polymerase Chain Reaction, Small Interfering RNA

Journal: OncoTargets and therapy

Article Title: Upregulated LAMB3 increases proliferation and metastasis in thyroid cancer

doi: 10.2147/OTT.S149613

Figure Lengend Snippet: Knockdown of LAMB3 gene inhibited migration and invasion in PTC cell lines. Notes: Migration assay: ( A ) migrating cell number was much less in TPC1, BCPAP, and KTC-1 cells transfected with siRNA compared with NC; ( B ) relative quantification of migration cell number. Invasion assay: ( C ) invading cell number was much less in TPC1, BCPAP, and KTC-1 cells transfected with siRNA compared with NC; ( D ) relative quantification of invasion cell number. Columns represent the mean of cell numbers from at least three independent experiments, and the little vertical bars at the top of the columns represent SD. *** P <0.01 in comparison with NC using Student’s t -test. Abbreviations: NC, negative control; PTC, papillary thyroid cancer; siRNA, small interfering RNA.

Article Snippet: Small interfering RNA (siRNA) for LAMB3 and nontargeting siRNA (negative control [NC] siRNA) were purchased from GenePharma (Shanghai, People’s Republic of China) for siRNA-mediated gene knockdown.

Techniques: Knockdown, Migration, Transfection, Quantitative Proteomics, Invasion Assay, Comparison, Negative Control, Small Interfering RNA

Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

Article Title: High Throughput Screening and QSAR-3D/CoMFA: Useful Tools to Design Predictive Models of Substrate Specificity for Biocatalysts †

doi: 10.3390/90800673

Figure Lengend Snippet: Microorganisms giving yields higher than 20% in the oxidation of cyclohexanol. [Cyclohexanol] =10 mM. Reaction time = 72 h. T = 28 ºC, Stirring speed = 250 rpm. [ 23 ]

Article Snippet: Only the fungi Coniochaeta velutina and Tetracladium setigerum display considerable activity without side-reactions. table ft1 table-wrap mode="anchored" t5 caption a7 Entry Phylum Microorganisms Reference OxidationYield (%) 1 Yeast Williopsis californica CBS 2158 94 2 Yeast Williopsis saturnus NCYC 2313 78 3 Bacterium Rhodococcus rhodochrous DSMZ 11097 68 4 Yeast Fellomyces sp CBS 8616 61 5 Yeast Pachysolen tannophilus NCYC 1597 60 6 Yeast Kluyveromyces lactis CBS 2896 56 7 Yeast Arthroascus fermentans CBS 7830 56 8 Yeast Sporidiobolus johnsonii NCYC 421 51 9 Bacterium Rhodococcus erythropolis DSMZ 8424 46 10 Yeast Chelatococcus asaccharovorans ATCC 51531 45 11 Yeast Pichia fermentans NCYC 1657 40 12 Filamentous Fungus Phialemonium curvatum CBS 505.82 38 13 Yeast Sporopachyderma cereana NCYC 856 37 14 Yeast Stephanoascus ciferii NCYC 2305 32 15 Bacterium Ralstonia eurotropha DSMZ 2839 32 16 Filamentous Fungus Corynascus novoguineensis IMI 291955 31 17 Marine Fungus Ceriosporopsis tubulifera ATCC 64283 27 18 Basidiomycete Irpex vellereus CBS 515.92 27 19 Yeast Trichosporon equatile NCYC 2635 25 20 Bacterium Cytophaga lytica DSMZ 2039 24 21 Filamentous Fungus Coniochaeta velutina CBS 981.68 23 22 Filamentous Fungus Tetracladium setigerum IMI 94061 23 23 Bacterium Pseudomonas sp DSMZ 7322 21 Open in a separate window Microorganisms giving yields higher than 20% in the oxidation of cyclohexanol.

Techniques: